Formation of Gefitinib-Resistant Lung Cancer Cell Lines and their Changes in Epidermal Growth Factor Receptor Signaling Pathway

Lung cancer is one of the top ten malignant tumors, seriously endangering people’s lives and health, with morbidity mortality ranking first. Molecular targeted therapy overcomes shortcomings traditional chemotherapy. In recent years, application tumor has become wider wider. Gefitinib most successful molecular drug for treatment lung in years. The purpose this article to explore establishment method gefitinib-resistant cell lines mechanism epidermal growth factor receptor signaling pathway change. gradually increasing concentration gefitinib was used successfully establish NC-H1985 gefitinibresistant cells. strains were also analyzed receptor, messenger ribonucleic acid expression at different times using anti-tubercular method. results show that line a certain difference compared parental cell. With extension culture time, decreased by 51 % 18S ribosomal acid, cycle threshold value increased 39 %. addition, higher relative cells, likely From 24 h 72 h, 37.5 After resistance cells 44.1 resting phase, 36.9 1 11.4 synthesis 4.3 2 phase 3.3 mitotic phase.